2004 Fiscal Year Final Research Report Summary
Monitoring of CD15s Ag in Clinical Renal Tx and xenogereic model.
| Project/Area Number |
15591717
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Urology
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
ISHIDA Hideki TWMU, Dept of Urology, Clinical fellow, 医学部, 助手 (60246543)
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| Co-Investigator(Kenkyū-buntansha) |
TANABE Kazunari TWMU, Dept of Urology, Assist Professor, 医学部, 助教授 (80188359)
MIYAMOTO Naoshi TWMU, Dept of Urology, Clinical fellow, 医学部, 助手 (50366332)
SHIRAKAWA Hiroki TWMU, Dept of Urology, Clinical fellow, 医学部, 助手 (60263539)
TOMA Hiroshi TWMU, Dept of Urology, Professor, 医学部, 教授 (90075549)
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| Project Period (FY) |
2003 – 2004
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| Keywords | CD15s antigen / blood type incompatible / Renal Transplantation / Xonotransplantation / immuno suppressive regimen |
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| Research Abstract |
Renal transplantation across the blood barrier is a unique model for investigating the humoral response to different carbohydrate antigens. However, in such a renal transplantation, the characteristics of B cells as well as of the antibodies produced by B cells are less well defined.
In the present study we investigated B cell subsets, i.e., the CD5(+) B-1 and CD5(-) B-2 subsets, by flow cytometric analysis, and their subclasses of antibody, by ELISA, in patients who had undergone renal transplantation across the blood barrier. The subjects consisted of five recipients with good function (group 1) and five recipients with graft loss (group 2) accompanied by antibody-titer elevation after ABO-incompatible renal transplantation.
The B-cell population analysis revealed that CD5(+) B-1 cells temporarily increased in all patients in both groups soon after transplantation, and that CD5(-) B-2 cells significantly increased one month after transplantation only in group 2. The antibody subclasses analysis showed mild elevation of IgG2 and IgM in group 1 as opposed to remarked elevation of IgG2,IgM and IgG1 in group 2.
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