2017 Fiscal Year Final Research Report
Regulation of proliferation and differentiation of quiescent neural stem cells in the adult brain
| Project/Area Number |
15H02349
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
HARIMA Yukiko
KAISE Takashi
SUEDA Risa
PIAO Wenhui
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 神経幹細胞 / 成体脳 / 静止状態 / 発現振動 / 光遺伝学 |
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| Outline of Final Research Achievements |
We performed slice cultures of the adult brain of Hes1 reporter mice and found that Hes1 is steadily expressed at high levels in quiescent neural stem cells. It is likely that this steady Hes1 expression may repress Mash1 expression. Because Mash1-negative neural stem cells do not proliferate well, we next tried to induce Mash1 oscillations optogenetically. The Mash1-inducible system was introduced with lentivirus into neural stem cells in the adult mouse brain, and oscillatory expression was induced by light illumination. After one week, we found that many neural stem cells were activated and produced new neurons.
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| Free Research Field |
神経科学
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