Before and after the neck: to understand the vertebrate body elaboration from the perspective of muscle, skeleton, and nerve development

2017 Fiscal Year Annual Research Report

• Project/Area Number: 15H02416
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: 倉谷 滋 国立研究開発法人理化学研究所, 倉谷形態進化研究室, 主任研究員 (00178089)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 形態進化 / 脊椎動物 / 比較発生学 / 実験発生学

Outline of Annual Research Achievements

本研究では、複数の脊索動物種を用い、筋肉と骨格の前駆細胞の挙動と胚環境、その背景にある遺伝子相互作用を比較し、筋・骨格・神経系の進化シナリオを復元することを目指す。平成29年度は、体節の一部、皮筋節に由来しながら、遠位方向に長距離を移動する筋芽細胞（migratory muscle precursor; MMP）に注目し、MMPから形成される四肢筋、舌筋、横隔膜の骨格筋の分化制御について解析した。羊膜類のMMPの発生には、Pax3遺伝子、Lbx1遺伝子による筋分化と細胞移動の制御が必要である。円口類ヤツメウナギは四肢（対鰭）を持たないが、舌筋の相同物である鰓下筋を持つ。本年度のヤツメウナギを用いた実験では、遺伝子欠失個体の作成とゲノム配列解析により、Lbx遺伝子が鰓下筋前駆細胞の咽頭部への伸長に必須であることが示唆された。また、硬節および鰓弓骨格のマーカーとしてScxを単離し、その発現領域と筋前駆細胞の位置を比較した。軟骨魚類トラザメを用いた解析では、遺伝子重複により二つのLbx遺伝子が存在し、骨格筋の発生において相互排他的なパターンで発現することが示された。特に鰓下筋においては前方と後方の筋組織（哺乳類のオトガイ舌骨筋、胸骨舌骨筋に相同）がそれぞれ異なる発生遺伝子に制御されて、それらが結合する骨格や神経系との連携を生じながら、発生後期に分化することが示唆された。また、トラザメのLbx遺伝子群は対鰭筋、僧帽筋および体壁筋にも発現し、大きく異なるこれらの筋の分化タイミングに対応する発現パターンを示した。これらの知見から、脊椎動物の初期進化において、皮筋節の腹側が二次的に進展して体幹部腹側を覆う発生機構が獲得され、その吻側部分である頸部筋の複雑化にLbxの発生制御遺伝子の重複が関わったことが示唆された。

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Uppsala University(スウェーデン)
  • Country Name: SWEDEN
  • Counterpart Institution: Uppsala University
• [Int'l Joint Research] Academia Sinica(台湾)
  • Country Name: その他の国・地域
  • Counterpart Institution: Academia Sinica
• [Int'l Joint Research] Chinese Academy of Sciences(中国)
  • Country Name: CHINA
  • Counterpart Institution: Chinese Academy of Sciences
• [Int'l Joint Research] University of Essex(英国)
  • Country Name: UNITED KINGDOM
  • Counterpart Institution: University of Essex
• [Int'l Joint Research] Universidad de Navarra(スペイン)
  • Country Name: SPAIN
  • Counterpart Institution: Universidad de Navarra
• [Journal Article] Hagfish and lamprey Hox genes reveal conservation of temporal colinearity in vertebrates (2018)
  • Author(s): Pascual-Anaya J, Sato I, Sugahara F, Higuchi S, Paps J, Ren Y, Takagi W, Ruiz-Villalba A, Ota KG, Wang W, Kuratani S.
  • Journal Title: Nat Ecol Evol. Volume: 2 Pages: 859-866
  • DOI: 10.1038/s41559-018-0526-2.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expansions, diversification, and interindividual copy number variations of AID/APOBEC family cytidine deaminase genes in lampreys (2018)
  • Author(s): Holland SJ, Berghuis LM, King JJ, Iyer LM, Sikora K, Fifield H, Peter S, Quinlan EM, Sugahara F, Shingate P, Trancoso I, Iwanami N, Temereva E, Strohmeier C, Kuratani S, Venkatesh B, Evanno G, Aravind L, Schorpp M, Larijani M, Boehm T.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 3：115 Pages: E3211-E3220
  • DOI: 10.1073/pnas.1720871115
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Development of hypobranchial muscles with special reference to the evolution of the vertebrate neck. (2018)
  • Author(s): Adachi N, Pascual-Anaya J, Hirai T, Higuchi S, Kuratani S.
  • Journal Title: Zoological Lett. Volume: 4 Pages: 5
  • DOI: 10.1186/s40851-018-0087-x
  • Peer Reviewed / Open Access
• [Journal Article] The neural crest and evolution of the head/trunk interface in vertebrates. (2018)
  • Author(s): Kuratani S, Kusakabe R, Hirasawa T.
  • Journal Title: Dev Biol. Volume: S0012-1606 Pages: 30922-30923
  • DOI: 10.1016/j.ydbio.2018.01.017.
  • Peer Reviewed / Open Access
• [Journal Article] Evolution of the vertebrate neurocranium: problems of the premandibular domain and the origin of the trabecula. (2018)
  • Author(s): Kuratani S, Ahlberg PE.
  • Journal Title: Zoological Lett. Volume: 4 Pages: 1
  • DOI: 10.1186/s40851-017-0083-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Metamerism in cephalochordates and the problem of the vertebrate head. (2017)
  • Author(s): Onai T, Adachi N, Kuratani S.
  • Journal Title: Int J Dev Biol Volume: 61 Pages: 621-632
  • DOI: 10.1387/ijdb.170121to
  • Peer Reviewed
• [Journal Article] Anatomical integration of the sacral-hindlimb unit coordinated by GDF11 underlies variation in hindlimb positioning in tetrapods. (2017)
  • Author(s): Matsubara Y, Hirasawa T, Egawa S, Hattori A, Suganuma T, Kohara Y, Nagai T, Tamura K, Kuratani S, Kuroiwa A, Suzuki T.
  • Journal Title: Nat Ecol Evol. Volume: 1 Pages: 1392-1399
  • DOI: 10.1038/s41559-017-0247-y
  • Peer Reviewed
• [Journal Article] Migratory appendicular muscles precursor cells in the common ancestor to all vertebrates. (2017)
  • Author(s): Okamoto E, Kusakabe R, Kuraku S, Hyodo S, Robert-Moreno A, Onimaru K, Sharpe J, Kuratani S, Tanaka M.
  • Journal Title: Nat Ecol Evol Volume: 1 Pages: 1731-1736
  • DOI: 10.1038/s41559-017-0330-4
  • Peer Reviewed
• [Journal Article] Constrained vertebrate evolution by pleiotropic genes. (2017)
  • Author(s): Hu H, Uesaka M, Guo S, Shimai K, Lu TM, Li F, Fujimoto S, Ishikawa M, Liu S, Sasagawa Y, Zhang G, Kuratani S, Yu JK, Kusakabe TG, Khaitovich P, Irie N; EXPANDE Consortium.
  • Journal Title: Nat Ecol Evol. Volume: 1 Pages: 1722-1730
  • DOI: 10.1038/s41559-017-0318-0
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Nitric Oxide regulates mouth development in amphioxus. (2017)
  • Author(s): Annona G, Caccavale F, Pascual-Anaya J, Kuratani S, De Luca P, Palumbo A, D'Aniello S.
  • Journal Title: Sci Rep Volume: 7 Pages: 8432
  • DOI: 10.1038/s41598-017-08157-w
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Differing contributions of the first and second pharyngeal arches to tympanic membrane formation in the mouse and chick. (2017)
  • Author(s): Furutera T, Takechi M, Kitazawa T, Takei J, Yamada T, Vu Hoang T, Rijli FM, Kurihara H, Kuratani S, Iseki S
  • Journal Title: Development. Volume: 144 Pages: 3315-3324
  • DOI: 10.1242/dev.149765.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Expression patterns of Sema3A in developing amniote limbs: With reference to the diversification of peripheral nerve innervation. (2017)
  • Author(s): Noguchi K, Ishikawa R, Kawaguchi M, Miyoshi K, Kawasaki T, Hirata T, Fukui M, Kuratani S, Tanaka M, Murakami Y.
  • Journal Title: Dev Growth Differ Volume: 59 Pages: 270-285
  • DOI: 10.1111/dgd.12364
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Outflow tract septation and the aortic arch system in reptiles: lessons for understanding the mammalian heart. (2017)
  • Author(s): Poelmann RE, Gittenberger-de Groot AC, Biermans MWM, Dolfing AI, Jagessar A, van Hattum S, Hoogenboom A, Wisse LJ, Vicente-Steijn R, de Bakker MAG, Vonk FJ, Hirasawa T, Kuratani S, Richardson MK.
  • Journal Title: Evodevo Volume: 10 Pages: 8:9
  • DOI: 10.1186/s13227-017-0072-z.
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Reconstructing the ancestral vertebrate brain. (2017)
  • Author(s): Sugahara F, Murakami Y, Pascual-Anaya J, Kuratani S.
  • Journal Title: Dev Growth Differ. Volume: 59 Pages: 163-174
  • DOI: 10.1111/dgd.12347
  • Peer Reviewed / Open Access
• [Presentation] “Does the ontogeny recapitulate phylogeny?“（個体発生は系統発生を繰り返すのか？) (2017)
  • Author(s): Shigeru Kuratani
  • Organizer: 50th Annual Meeting for the Japanese Society of Developmental Biologists
  • Int'l Joint Research / Invited
• [Presentation] Acquistion of The Turtle Shell: Changes in Developmental Program Behind The Evolutionary Novelty (2017)
  • Author(s): Shigeru Kuratani
  • Organizer: 18th International Congress of Developmental Biology
  • Int'l Joint Research / Invited
• [Presentation] かたちの進化と発生 (2017)
  • Author(s): 倉谷 滋
  • Organizer: 名古屋大学EGER グリーン自然科学レクチャー 2017
  • Invited
• [Remarks] 研究所HP
  • URL: http://www.riken.jp/research/labs/chief/evol_morphol/
• [Remarks] 研究室HP
  • URL: http://www.cdb.riken.jp/emo/japanese/indexj.html