Application of novel mass spectrometric techniques for highly sensitive, specific and speedy identification of new psychoactive substances in human specimens

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H02530
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Legal medicine
• Research Institution: Nagoya University
• Principal Investigator: Ishii Akira 名古屋大学, 医学系研究科, 教授 (30252175)
• Co-Investigator(Kenkyū-buntansha): 那須 民江 中部大学, 生命健康科学部, 教授 (10020794) ; 林 由美 名古屋大学, 医学系研究科(保健), 助教 (30632707) ; 財津 桂 名古屋大学, 医学系研究科, 准教授 (30700546) ; 土橋 均 名古屋大学, 医学系研究科, 招へい教員 (40596029) ; 妹尾 洋 愛知医科大学, 医学部, 教授 (50236113) ; 小川 匡之 愛知医科大学, 医学部, 講師 (50559937) ; 草野 麻衣子 名古屋大学, 医学系研究科, 特任助教 (60733574) ; 内藤 久雄 藤田保健衛生大学, 医学部, 講師 (90547556)
• Research Collaborator: HISATSUNE KAZUAKI 愛知県科学捜査研究所, 化学鑑定室, 警察職員 ; NAKAJIMA JUN'ICHI 東京都健康安全研究センター, 生薬研究室, 主任研究員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 法中毒学 / 法医学 / 質量分析 / 危険ドラッグ / 合成カンナビノイド / 飛行時間型質量分析計

Outline of Final Research Achievements

The aim of this project is to develop simple and comprehensive methods for determining novel abused drugs including synthetic cannabinoids by combining ultra-sensitive and/or highly specific mass spectrometers, and conventional mass spectrometers. We developed a simple method for determining several synthetic cannabinoids and their metabolites using a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer (LC-QTOFMS). The combination of an LC-QTOFMS and an LC-QTrap enabled the first identification of 5-fluoro-ADB (5-F-ADB) in the blood of a fatal intoxication case of 5-F-ADB and diphenidine. We then proposed possible metabolic pathways of the compounds. We are now investigating the difference of 5-F-ADB levels among organs in which the expression levels of carboxyesterase differ.

Free Research Field

法医学