Cooperative effects of natural-derived lipids and membrane proteins in lipid bilayer membranes

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03768
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Toyohashi University of Technology
• Principal Investigator: Tero Ryugo 豊橋技術科学大学, 工学(系)研究科(研究院), 准教授 (40390679)
• Co-Investigator(Kenkyū-buntansha): 平野 愛弓 東北大学, 材料科学高等研究所, 教授 (80339241) ; 戸澤 譲 埼玉大学, 理工学研究科, 教授 (90363267)
• Research Collaborator: FUKUMOTO Kohei ; SUZUKI Yuya ; NIIYAMA Yuya ; OKAMOTO Yoshiaki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 脂質二重膜 / 膜タンパク質 / 原子間力顕微鏡 / 蛍光顕微鏡

Outline of Final Research Achievements

This project aimed to elucidate the active state created by a plurality of membrane proteins and surrounding lipid molecules, and relating phenomena using a cell membrane model system. We reconstructed ion channel proteins that were obtained from cultured cells and cell-free protein synthesis system, in supported lipid bilayers (SLBs). Distribution and molecular structures of lipids and membrane proteins were observed by fluorescence microscopy and atomic force microscopy. We propose a model to explain the mechanism of reconstitution by clarifying the role of cell membrane derived components in SLB. We revealed that the role of intra-membrane microdomains in the reconstitution process. It is revealed that the molecular orientation of the channel protein expressed by cell-free synthesis varies depending on the reconstitution process.

Free Research Field

界面物理化学