Development of an analytical system for quantitative and qualitative variation of miRNA

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H03830
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Tokyo University of Pharmacy and Life Science
• Principal Investigator: Umemura Tomonari 東京薬科大学, 生命科学部, 教授 (10312901)
• Co-Investigator(Kenkyū-buntansha): 谷 英典 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (10635329) ; 手嶋 紀雄 愛知工業大学, 工学部, 教授 (30292501) ; 青木 元秀 東京薬科大学, 生命科学部, 助教 (30418917) ; リム リーワ 岐阜大学, 工学部, 准教授 (80377689)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: クロマトグラフィー / モノリスカラム / 機能性RNA / マイクロリアクター

Outline of Final Research Achievements

In order to quantitatively analyze non-coding RNAs including micro RNAs, mass spectrometry-based separation system was constructed. We have refined our monolithic column technology and produced a variety of capillary-based analytical tools for chromatographic separation, analyte extraction, and catalytic reaction. We have also developed interface devices for optimal coupling of separation techniques with mass spectrometry. We have integrated such analytical tools, devices and instruments to construct highly-efficient analysis system for the evaluation of quantitative and qualitative variation of non-coding RNAs.

Free Research Field

分析化学