Development of a screening technique of protein-protein interaction inhibitors in the cytosolic environment

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04190
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: The University of Tokyo
• Principal Investigator: KAWAHARA MASAHIRO 東京大学, 大学院工学系研究科(工学部), 准教授 (50345097)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 蛋白質 / 創薬 / 遺伝子 / スクリーニング / シグナル伝達

Outline of Final Research Achievements

This study is directed to develop a technique to easily detect protein-protein interactions in the cytosolic environment as well as to enable inhibitor screening. To attain these, two kinds of signaling proteins are utilized to design a system that converts protein-protein interactions into growth signals as readout. Consequently, we successfully detect protein-protein interactions with cell growth and evaluate the inhibitors.

Free Research Field

細胞工学