Evaluation of human iPSCs by incomplete reprogramming and genome instability

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04278
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Aichi Medical University
• Principal Investigator: Okada Yohei 愛知医科大学, 医学部, 准教授 (30383714)
• Co-Investigator(Renkei-kenkyūsha): OKANO HIDEYUKI 慶應義塾大学, 医学部, 教授 (60160694) ; MIYA FUYUKI 東京医科歯科大学, 医学部, 助教 (50415311) ; IKADA EIJI 山口大学, 医学部, 教授 (30232177) ; YASUDA MUNEYOSHI 愛知医科大学, 医学部, 准教授 (10440752)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ヒトiPS細胞 / 造腫瘍性 / ゲノム不安定性 / 不完全なリプログラミング

Outline of Final Research Achievements

Induced pluripotent stem cells (iPSCs) hold great promise for regenerative medicine and in vitro modeling of human diseases. To overcome their abnormal differentiation and tumorigenicity, the method to evaluate bona fide “appropriately reprogrammed human iPSCs” has been expected. In this study, by focusing on the differences of the tumorigeniticy (glioma-like tumor formation) among human iPSC clones caused by the incomplete reprogramming and genomic instability, we identified reprogramming recalcitrant genes as “Score card”, and examined their applicability to the evaluation of human iPSCs.

Free Research Field

分子神経生物学、幹細胞生物学