Suppression of hepatocarcinogeneis based on the elucidation of HBV integration and epigenome alterations analyzed by G-NaVI

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04304
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: Itoh Fumio 聖マリアンナ医科大学, 医学部, 教授 (90223180)
• Co-Investigator(Kenkyū-buntansha): 山本 博幸 聖マリアンナ医科大学, 医学部, 准教授 (40332910) ; 渡邊 嘉行 聖マリアンナ医科大学, 医学部, 講師 (90329243)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: エピゲノム / HBV / 肝癌 / DNA組み込み / DNAメチル化

Outline of Final Research Achievements

Integration of DNA viruses into the human genome plays an important role in various types of tumors, including hepatitis B virus (HBV)-related hepatocellular carcinoma. To gain clearer insights into the roles of HBV DNA integration in HCC, we have characterized the molecular details and clinical impact of HBV integration on the epigenomes of human cells and HBV. We have also successfully developed a single-molecule, real-time sequencing-based method for structural analysis of integrated HBV genomes. The observed dynamic changes in DNA methylation of the host and viral genomes as well as genetic and epigenetic alterations in HCC may functionally affect the biological behavior of HBV-related HCC.

Free Research Field

消化器内科学