Establishment of next-generation CRISPR system for human functional genomics

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04319
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genome biology
• Research Institution: The University of Tokyo
• Principal Investigator: Ui-Tei Kumiko 東京大学, 大学院理学系研究科(理学部), 准教授 (50213327)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: CRISPR / dCas9 / base-pairing / thermodynamics

Outline of Final Research Achievements

This study aims to develop genome editing technology of next generation for human functional genomics by improving CRISPR method. The CRISPR system is a new genome editing technology based on a prokaryotic acquired immune system, and has attracted attention as a breakthrough technology applicable to all kinds of organisms. In the CRISPR system, a guide RNA that distinguishes a target genomic site with a complementary base sequence forms a complex with a Cas9 protein, and Cas9 cleaves a genomic region where the guide RNA forms base-pairing. Therefore, in this study, using dCas9 which has no genome cleavage activity, we succeeded in establishing a practical CRISPR technique with high genome editing efficiency by combining experimental method and bioinformatics method.

Free Research Field

ゲノム生物学