Verification of unit raft hypothesis by high-resolution single-molecule imaging

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04351
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Gifu University (2017); Kyoto University (2015-2016)
• Principal Investigator: Suzuki Kenichi 岐阜大学, 研究推進・社会連携機構, 教授 (50423059)
• Co-Investigator(Renkei-kenkyūsha): ANDO Hiromune 岐阜大学, 研究推進・社会連携機構, 教授 (20372518)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ラフト / １分子観察 / GPIアンカー型受容体 / 糖脂質 / シグナル伝達

Outline of Final Research Achievements

In this study, we examined the hypothesis that raft-associated glycolipids and GPI-anchored receptors (GARs) form homodimers via specific interactions, and work as a unit for downstream signal transduction.
We found that a variety of glycolipids and GARs formed homodimers via specific interactions, which can be generalized. We also found that the homodimers of glycolipids and GARs were stabilized by lipid interactions. Furthermore, our results suggest that these homodimers work as a unit for inducing downstream signaling events or for regulating activity of membrane receptors.

Free Research Field

細胞生物物理学