Prion protein binds to aldolase A of bovine intestinal M cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04586
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Animal production science
• Research Institution: Tohoku University
• Principal Investigator: Aso Hisashi 東北大学, 農学研究科, 教授 (50241625)
• Co-Investigator(Kenkyū-buntansha): 北澤 春樹 東北大学, 農学研究科, 准教授 (10204885) ; 奥 夏美 (岡田夏美) 東北大学, 農学研究科, 技術一般職員 (10621584) ; 野地 智法 東北大学, 農学研究科, 准教授 (10708001) ; 渡邊 康一 東北大学, 農学研究科, 助教 (80261494)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: プリオン / M細胞 / アルドラーゼA / ウシ腸管上皮細胞 / マウス腸管上皮細胞 / 侵入 / トランスサイトーシス / 抗体阻害

Outline of Final Research Achievements

BSE is a fatal neurodegenerative disorder in cattle and is linked to variant Creutzfeldt-Jakob disease in humans. We show that M cells can incorporate large numbers of PrP coated magnetic particles into intracellular vesicles, which we collected. The results of 2-DE show a specific protein associated with the PrP-coated particles. This protein was identified as aldolase A using LC-MS/MS analysis. The binding of rbPrP to aldolase A was inhibited by pre-treatment of anti-aldolase A antibody. We established the aldolase-knockout cell line (MIE ALDOA -/- cells) by TALEN method. After the differentiation for M cell, MIE ALDOA -/- cells lacked the ability of transporting PrP coated magnetic particles into the basal media through the intracellular vesicles.
These results suggest that bovine M cells synthesize aldolase A, and that aldolase A-positive M cells may play a key role in the invasion of BSE into the body.

Free Research Field

細胞生物学