2017 Fiscal Year Final Research Report
Analysis of mRNA fates with exclusive function mediated by RNA granules
| Project/Area Number |
15H04628
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
濱田 隆宏 東京大学, 大学院総合文化研究科, 助教 (20452534)
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| Co-Investigator(Renkei-kenkyūsha) |
Sato Masanao 北海道大学, 大学院農学研究科, 助教 (20517693)
Okamoto Akimitsu 東京大学, 先端科学技術研究センター, 教授 (60314233)
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 植物 / mRNA制御 / 環境応答 / 高温ストレス / ストレス顆粒 / P-ボディー / RNA顆粒 / シロイヌナズナ |
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| Outline of Final Research Achievements |
We have studied dynamic changes of P-bodies and stress granules (SG) in Arabidopsis before and after heat stress application. DCP2 (decapping enzyme 2; P-body marker) began to accumulate to cytoplasmic granules preformed by DCP1, while eIF4A2 (SG marker) started to form granules independently from DCP1/2. SG often came close to P-bodies over time, and some time later it detached the contact with P-bodies. We also analyzed the RNA species of which levels were elevated and those lessened after temperature rises. It turned out that almost 7000 RNA species increased and almost the same number of RNA species decreased by heat stress. It meant that 30% of total genes were transcriptionally activated while another 30% of mRNAs were specifically destabilized. When the temperature returned normal, about half member of each gene set showed reciprocal pattern in accumulation. The phosphorylation level of DCP1 reflects the rise of temperature and is likely to be involved in DCP2 accumulation.
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| Free Research Field |
植物分子生物学・環境応答論
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