Studies on the efficacy of toxin neutralization and safety of Shiga toxin-specific secretory IgA type plantibody produced by practical plants

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04660
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental and hygienic pharmacy
• Research Institution: University of Shizuoka
• Principal Investigator: Imai Yasuyuki 静岡県立大学, 薬学部, 教授 (80160034)
• Co-Investigator(Kenkyū-buntansha): 黒羽子 孝太 静岡県立大学, 薬学部, 講師 (90333525)
• Co-Investigator(Renkei-kenkyūsha): Kobayashi Hirokazu 静岡県立大学, 大学院 食品栄養環境科学研究院, 教授 (80170348) ; Niwa Yasuo 静岡県立大学, 大学院 食品栄養環境科学研究院, 助教 (00222191)
• Research Collaborator: NAKANISHI Katsuhiro ; AKIMOTO Yoshihiro ; KAWAKAMI Hayato ; MATSUBARA Sachie
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 抗体医薬 / 植物発現系 / 粘膜免疫 / 免疫グロブリンA / 微生物

Outline of Final Research Achievements

Transgenic plants that produce secretory immunoglobulin A (SIgA) specific to Shiga toxin 1 (Stx1) were developed using a model plant (Arabidopsis thaliana) and a vegetable (Lactuca sativa). Considering the initial entry of Stx1 can occur through cytotoxicity to colon epithelial cells, we established an assay using a human colon cell line, Caco-2. Plant-made IgA neutralized the toxicity of Stx1 against Caco-2 cells. Production of secretory component (SC), a component of SIgA, was increased in the transgenic plants by the addition of an endoplasmic reticulum retention signal to SC. When plant-made SC was administered to mice, anti-SC antibodies were induced by intravenous administration while no such antibodies were made by oral administration. This result provides evidence as to the safety of oral application of plant-made biologics.

Free Research Field

免疫学、微生物学