Clarification of transcriptional network that drives T cell lineage specification

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04743
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: kawamoto hiroshi 京都大学, ウイルス・再生医科学研究所, 教授 (00343228)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 多能前駆細胞 / 系列決定 / 転写因子 / エピジェネティクス

Outline of Final Research Achievements

We aimed to clarify the molecular mechanisms of the step by which myeloid-T-B progenitors are committed to myeloid-T progenitors. In 2015, we reported the time course data of this process as a part of FANTOM5 project conducted by RIEKN（Science, 329:1014, 2015）, and also succeeded in developing an on-off induction culture system of multipotent hematopoietic　progenitors（Stem Cell Reports, 2015）. In 2016, we reported that T cell progenitors are converted into B lineage cells upon inactivation of polycomb complex（Gene Dev, 2016）. In one of planned subjects, in which we knocked out snai1 and snai2, which had been listed up from FANTOM5 project as candidate genes that play role in early T cell differentiation, we produced T cell specific conditional double knockout mice, but unfortunately they did not show phenotypes. However, we have achieved publications as mentioned above along with the aim of this study, and so, as a total, we think that the results were satisfactory.

Free Research Field

免疫学　血液学