Elucidation of the pathology and treatment of refractory small intestinal ulcer disease using genetically engineered mouse and PG mass spectrometry

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04811
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Kyorin University
• Principal Investigator: HISAMATSU TADAKAZU 杏林大学, 医学部, 教授 (60255437)
• Co-Investigator(Kenkyū-buntansha): 杉浦 悠毅 慶應義塾大学, 医学部(信濃町), 特任講師 (30590202) ; 金井 隆典 慶應義塾大学, 医学部(信濃町), 教授 (40245478)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 小腸潰瘍 / プロスタグランジン / SLCO2A1 / CEAS

Outline of Final Research Achievements

For the diagnosis of a patient with a CEAS patient, a simple diagnostic technique by SLCO 2A1 protein fluorescent immunostaining method was established. For functional analysis of SLCO2A1 mutant protein found in patients, a gene transfer system by Xenopus oocytes was set up and as a result of the transport experiment of PG, Mutations that completely lost function and mutations that only partially declined in function were observed. Furthermore, it was shown that female sex hormone influences PG transporting ability. We established vascular endothelial specific SLCO2A1 deficient mice and established a mass spectrometry method of PG metabolism analysis system in mouse small intestine. As a result, changes in PG metabolism in each small intestinal ulcer model including genetically modified mice could be observed.

Free Research Field

下部消化管　小腸潰瘍