Analysis of LINE-1 retrotransposition using poly(I:C) animal model

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04891
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: Kumamoto University
• Principal Investigator: Iwamoto Kazuya 熊本大学, 大学院生命科学研究部(医), 教授 (40342753)
• Co-Investigator(Renkei-kenkyūsha): BUNDO Miki 熊本大学, 大学院生命科学研究部, 准教授 (00597221) ; KATO Tadafumi 理化学研究所, 脳科学総合研究センター, チームリーダー (30214381) ; KASAI Kiyoto 東京大学, 大学院医学系研究科, 教授 (80322056)
• Research Collaborator: MURATA Yui 熊本大学, 大学院生命科学研究部, 博士研究員 (10802077) ; SAWAMURA Rie 熊本大学, 大学院生命科学研究部, 助教 (80509654) ; KIYOTA Emi 熊本大学, 大学院生命科学研究部, 技術補佐員 ; UCHINUNO Emi 熊本大学, 大学院生命科学研究部, 技術補佐員 ; UEDA Junko 理化学研究所, 脳科学総合研究センター, 技術補佐員
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 体細胞変異 / 統合失調症

Outline of Final Research Achievements

Using poly (I:C) mouse model, we analyzed fetal neural progenitor cells. RNA-seq analysis revealed increased expression of immune response and inflammation-related genes and decreased expression of genes related to neurodevelopment. Protein analysis by iTRAQ showed increased expression of factors suppressing viral activity. In addition, analysis of the cytosine modification status for each of the three active LINE-1 subfamilies in mice identified subfamily dependent epigenetic changes. One subfamily showed decreased cytosine methylation and increased hydroxymethylation, suggesting the possibility of selective activation of retrotransposition activity.

Free Research Field

精神医学