Elucidation of reoxygenation and energy metabolism in radiotherapy for clinical application

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H04908
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Radiation science
• Research Institution: Gifu University
• Principal Investigator: Matsuo Masayuki 岐阜大学, 大学院医学系研究科, 教授 (40377669)
• Co-Investigator(Kenkyū-buntansha): 村井 太郎 名古屋市立大学, 大学院医学研究科, 助教 (00747602) ; 芝本 雄太 名古屋市立大学, 大学院医学研究科, 教授 (20144719) ; 河合 辰哉 名古屋市立大学, 大学院医学研究科, 研究員 (70597822) ; 杉江 愛生 名古屋市立大学, 大学院医学研究科, 准教授 (80509258)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 放射線治療 / 再酸素化 / 解糖系代謝

Outline of Final Research Achievements

To examine the relationship between local oxygen partial pressure and energy metabolism in the tumor, electron paramagnetic resonance imaging (EPRI) and magnetic resonance imaging (MRI) with hyperpolarized [1-13C] pyruvate were performed.
SCCVII and HT29 solid tumors were imaged by EPRI and 13C-MRI using hyperpolarized [1-13C] pyruvate. The effect of a single dose of 5 Gy irradiation on the pO2 and metabolism was also investigated by sequential imaging of EPRI and 13C-MRI in HT29 tumors. In vivo studies revealed SCCVII tumor had a significantly larger hypoxic fraction compared to HT29 tumor. The flux of pyruvate-to-lactate conversion was also higher in SCCVII than HT29. The lactate-to-pyruvate ratio in hypoxic regions 24 hours after 5-Gy irradiation was significantly higher than those without irradiation in HT29 tumor. The in vitro study showed an increase in extracellular acidification rate after irradiation.

Free Research Field

放射線生物学