On the regulatory mechanism of hard tissue metabolism by 8-nitro-cGMP

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Showa University
• Principal Investigator: Kamijo Ryutaro 昭和大学, 歯学部, 教授 (70233939)
• Co-Investigator(Kenkyū-buntansha): 赤池 孝章 東北大学, 医学系研究科, 教授 (20231798) ; 宮本 洋一 昭和大学, 歯学部, 准教授 (20295132) ; 山田 篤 昭和大学, 歯学部, 講師 (50407558) ; 片桐 岳信 埼玉医科大学, 医学部, 教授 (80245802)
• Co-Investigator(Renkei-kenkyūsha): TANAKA Sakae 東京大学, 医学系研究科, 教授 (50282661)
• Research Collaborator: SUZUKI Dai 昭和大学, 歯学部, 講師 (00585797) ; YOSHIMURA Kentaro 昭和大学, 歯学部, 助教 (10585699) ; FUNATO Sakie 昭和大学, 歯学部, 助教 (20783252) ; IZUMIDA Eri 昭和大学, 歯学部, 助教 (70783497)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 軟骨細胞 / 骨芽細胞 / 破骨細胞 / 一酸化窒素 / 活性酸素種

Outline of Final Research Achievements

We investigated the production and functions of 8-nitro-cGMP, a novel second messenger of nitric oxide and reactive oxygen species, in the cartilage and bone tissues. It had been accepted that cGMP is a mediator of bone growth. In this study, we found that not only cGMP but also 8-nitro-cGMP was produced in chondrocytes in the growth plate cartilage. In addition, 8-nitro-cGMP promoted the proliferation of chondrocytes in the growth plates, which caused enhancement in bone growth. Secondly, we found that production of 8-nitro-cGMP in osteoclast precursor cells after exposure to inflammatory cytokines. Since 8-nitro-cGMP was found to promote osteoclast differentiation, it is suggested that inflammatory bone resorption is explained, at least in part, by increased production of 8-nitro-cGMP under inflammatory conditions.

Free Research Field

生化学