Usefulness of the complex of the three regenerative elements as a therapeutic agent for regeneration against endodontic diseases

2017 Fiscal Year Final Research Report

• Project/Area Number: 15H05022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Conservative dentistry
• Research Institution: Hiroshima University
• Principal Investigator: Shiba Hideki 広島大学, 医歯薬保健学研究科(歯), 教授 (60260668)
• Co-Investigator(Kenkyū-buntansha): 鈴木 茂樹 広島大学, 病院(歯), 講師 (30549762) ; 小武家 誠司 広島大学, 医歯薬保健学研究科(歯), 助教 (50744794) ; 永安 慎太郎 広島大学, 医歯薬保健学研究科(歯), 助教 (60635192) ; 本山 直世 広島大学, 医歯薬保健学研究科(歯), 助教 (70509661)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 歯内療法 / 再生3要素 / 細胞機能調節因子 / 宿主細胞傷害性 / LL37 / ヘパリン-LL37複合体 / 抗菌活性 / 抗炎症作用

Outline of Final Research Achievements

Cells, scaffolds and regulatory factors are the three key elements for tissue engineering. LL37 possesses broad-ranged bactericidal effects and angiogenesis ability, indicating LL37 is a candidate for a regulatory factor. However, LL37 is known to exhibit cytotoxic properties for host cells. Therefore, the development of LL37-complexes, by which the cytotoxicity for host cells is eliminated without antimicrobial abilities diminished, is essential for future clinical application. We demonstrated that LL37 (10 microM) premixed with heparin (2-8 microg/ml), one of glycosaminoglycans, did not show any cytotoxic effects but retained bactericidal effects. In addition, heparin-LL37 complex had cell-functioning ability. These findings suggest that the heparin-LL37 complex is a promising regulatory factor for regenerative therapy.

Free Research Field

歯内療法学