• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2017 Fiscal Year Final Research Report

Precise genomic correction by intercellular regulation of genome editing enzyme

Research Project

  • PDF
Project/Area Number 15H05581
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Medical genome science
Research InstitutionKyoto University

Principal Investigator

Hotta Akitsu  京都大学, iPS細胞研究所, 特定拠点講師 (50578002)

Project Period (FY) 2015-04-01 – 2018-03-31
Keywordsゲノム編集 / iPS細胞 / CRISPR / 相同組換え / 一塩基修復 / 一塩基変異
Outline of Final Research Achievements

About half of the genetic mutation causing human disease is a single nucleotide mutation. Deletion via NHEJ (nonhomologous end joining) can be relatively easily induced by using recent genomic editing techniques including CRISPR-Cas9, but knock-in using HDR (homologous recombination) efficiency was less than 1%, which was extremely inefficient. Therefore, we developed the CRONUS system in which CRISPR-Cas9 and guide-RNA were incorporated in the cells with a piggyBac transposon vector in advance, and Cas9 activity can be induced with two kinds of drugs. This made it possible to induce a desired single base modification at efficiencies as high as 20% to 30%, far exceeding the efficiency (<1%) so far.

Free Research Field

幹細胞遺伝子工学

URL: 

Published: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi