Mechanisms of anhydrobiosis-associated gene regulation in the cells and tissues of chironomid Polypedilum vanderplanki.

2017 Fiscal Year Annual Research Report

• Project/Area Number: 15H05622
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: グセフ オレグ 国立研究開発法人理化学研究所, 科学技術ハブ推進本部, マネージャー (30711999)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: anhydrobiosis / CAGE / cis-elements / tissue-specific / desiccation

Outline of Annual Research Achievements

CAGE libraries from dried and normal tissues of P.vanderplanki larvae were sequenced. The clustered readings were clustered, resulting in 13,727 promoter regions, of which 9573 were associated with 7876 protein-coding genes.We found of the tags in this group correspond to certain "anhydrobiotic genes", like PIMT methyltransferase or Trx-oxidoreductase. Finally, we created comprehensive atlas of tissue-specific cis-elements expression database for 4 main tissues of the annydrobiotic tissues. This unique resource is a platform for further development of technologies of dry preservation of cells and tissues. We found that genetic mechanism of tissue specificity and broad expression of protective genes across tissues is the strategy of anhydrobiosis and can be used for biomimetics

Research Progress Status

29年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

29年度が最終年度であるため、記入しない。

Research Products

• [Int'l Joint Research] Kazan Federal University(ロシア連邦)
  • Country Name: RUSSIA FEDERATION
  • Counterpart Institution: Kazan Federal University
• [Journal Article] Cooption of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki. (2018)
  • Author(s): Mazin P, Shagimardanova E4, Kozlova O4, Cherkasov A4, Sutormin R5, Stepanova VV6,2, Stupnikov A7, Logacheva M6,2,8, Penin A, Sogame Y, Cornette R, Tokumoto S., Miyata Y, Kikawada T, Gelfand MS, Gusev O
  • Journal Title: Proc Natl Acad Sci U S A. Volume: 115 Pages: E2477-E2486
  • DOI: 10.1073/pnas.1719493115
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Towards water-free biobanks: long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells (2017)
  • Author(s): S. Kikuta1 Shunsuke J. Watanabe, R.Sato, O. Gusev, A. Nesmelov, Y. Sogame, R.Cornette, T. Kikawada
  • Journal Title: Scientific Reports Volume: 7 Pages: 6540
  • DOI: 10.1038/s41598-017-06945-y
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] A new anhydrobiotic midge from Malawi, Polypedilum pembai sp.n. (Diptera: Chironomidae), closely related to the desiccation tolerant midge, Polypedilum vanderplanki Hinton (2017)
  • Author(s): R. Cornette, N. Yamamoto, M. Yamamoto, T. Kobayashi, N. Petrova, O. Gusev, S. Shimura, T. Kikawada, D. Pemba, T. Okuda
  • Journal Title: Systematic Entomology Volume: 42 Pages: 814-825
  • DOI: 10.1111/syen.12248
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Genetic background of enhanced radioresistance in an anhydrobiotic insect: transcriptional response to ionizing radiations and desiccation (2017)
  • Author(s): Ryabova Alina、Mukae Kyosuke、Cherkasov Alexander、Cornette Richard、Shagimardanova Elena、Sakashita Tetsuya、Okuda Takashi、Kikawada Takahiro、Gusev Oleg
  • Journal Title: Extremophiles Volume: 21 Pages: 109-120
  • DOI: 10.1007/s00792-016-0888-9
  • Peer Reviewed / Int'l Joint Research
• [Presentation] X-midge: cell and tissue-specific mechanisms of tolerance to complete desiccation in a unique insect. (2018)
  • Author(s): Oleg Gusev
  • Organizer: OIST International Seminar Series
  • Invited
• [Presentation] “Sleeping beauty” way to defeat aging: genomics of an anhydrobiotic insect (2018)
  • Author(s): Oleg Gusev
  • Organizer: International Conference "Interventions to extend healthspan and lifespan"
  • Int'l Joint Research / Invited
• [Presentation] Chromatin under extreme desiccation:3D organization of genome of an anhydrobiotic midge Polypedilum vanderplanki (2017)
  • Author(s): Oleg Gusev
  • Organizer: ConBio-2017
  • Int'l Joint Research
• [Presentation] Genetic background of extraordinary adaptivity of non-biting midges Chironomidae to extreme environmental conditions. (2017)
  • Author(s): Oleg Gusev
  • Organizer: 3nd Tokyo Vector Encounter
  • Int'l Joint Research / Invited