Development of novel long non-coding RNA mediated DNA methylation editting system

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06657
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Animal production science
• Research Institution: Hoshi University
• Principal Investigator: YAMAMOTO Naoki 星薬科大学, 先端生命科学研究所, 特任助教 (50757432)
• Research Collaborator: KINOSHITA Tetsu ; HATADA Izuho
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: エピゲノム編集 / DNAメチル化 / エピジェネティック / CRISPR/Cas9

Outline of Final Research Achievements

This study aims at establishing a technology that can specifically control the DNA methylation status of a specific genome region . A method using forced transcription of long noncoding RNA (200 bases or more), which do not translated into a protein, failed to obtain sufficient transcription efficiency and the expected effect was not obtained. On the other hand, using an artificial fusion gene obtained by fusing a DNA binding domain called dead Cas 9, which can be customized to recognize an arbitrary DNA sequence, and DNA-methylation-modifiing enzyme domain, I succeed to manipulate DNA methylation levels of a specific genome region with very high efficiency. The technology developed in this research is expected to greatly advance DNA methylation research over a wide range from basic to clinical research.

Free Research Field

エピゲノム編集