Development of new method for risk assessment of nanomaterials by concurrent analysis of uptake potential and toxicity using flowcytometer

2016 Fiscal Year Final Research Report

• Project/Area Number: 15H06893
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: National Institute of Occupational Safety and Health,Japan
• Principal Investigator: Toyooka Tatsushi 独立行政法人労働者健康安全機構労働安全衛生総合研究所, 産業毒性・生体影響研究グループ, 任期付研究員 (40423842)
• Research Collaborator: Miura Nobuhiko 独立行政法人労働者健康安全機構労働安全衛生総合研究所, 産業毒性・生体影響研究グループ, 上席研究員 (20229644) ; Wang Rui-sheng 独立行政法人労働者健康安全機構労働安全衛生総合研究所, 産業毒性・生体影響研究グループ, 上席研究員 (10321895) ; Ibuki Yuko 静岡県立大学, 品栄養環境科学研究院, 教授 (30236781)
• Project Period (FY): 2015-08-28 – 2017-03-31
• Keywords: 遺伝毒性 / ナノ毒性

Outline of Final Research Achievements

Establishment of a health risk-assessment method for nanomaterials is an urgent issue internationally. So far, we have developed a method for measure the intracellular uptake of nanoparticles using the side scattered light of the flow cytometer. This method canquickly and easily evaluate uptake potential of nanoparticles . On the other hand, although the method can evaluate uptake potential of nanoparticles, it is unknown what type of toxic effect is occurred. Therefore, in this study, we tried to develop a new nanomaterial evaluation method that can simultaneously analyze its toxic effects in addition to the intracellular uptake of nanomaterials.

Free Research Field

遺伝毒性学