Creation of novel aromatic-ring hydroxylating dioxygenase with extended substrate specificity toward polychlorinated biphenyls

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K00590
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Modeling and technologies for environmental conservation and remediation
• Research Institution: Kyoto Gakuen University (2016-2017); Yamagata University (2015)
• Principal Investigator: Sakurama Haruko 京都学園大学, バイオ環境学部, 講師 (90456426)
• Co-Investigator(Kenkyū-buntansha): 高塚 由美子 山形大学, 大学院理工学研究科, 助教 (70570810) ; 乾 秀之 神戸大学, バイオシグナル総合研究センター, 准教授 (90314509) ; 原 富次郎 山形大学, 大学院理工学研究科, 教授 (70616193)
• Project Period (FY): 2015-10-21 – 2018-03-31
• Keywords: ポリ塩化ビフェニル / 芳香環水酸化ジオキシゲナーゼ

Outline of Final Research Achievements

Polychlorinated biphenyls (PCBs) have been recognized as serious environmental pollutants on a global scale. In order to obtain novel aromatic-ring hydroxylating dioxygenases that can degrade on more highly chlorinated PCBs congeners, we engineered the aromatic-ring hydroxylating dioxygenase from Commamonas by a site-directed mutagenesis, and have successfully obtained a few mutants with extended substrate specificity. In addition, we have established the expression system of the two genes of the aromatic-ring hydroxylating dioxygenase with distinct substrate specificities.

Free Research Field

環境学