Biochemical analysis of Hybrid type polyketide synthase Steely that produce multi-products

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K01807
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biomolecular chemistry
• Research Institution: Sophia University
• Principal Investigator: SAITO Tamao 上智大学, 理工学部, 教授 (30281843)
• Co-Investigator(Kenkyū-buntansha): 森田 直樹 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ付 (60371085)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ポリケタイド / 生合成 / 微生物

Outline of Final Research Achievements

Polyketide synthase “Steely” has a distinct structure that is a fusion of a type I and III polyketide synthases. One of them, SteelyB was identified to be responsible for the production of DIF-1 that induces (pre)stalk cells differentiation in the slug stage of D. discoideum development. We identified LCC-1, chlorinated benzofuran that accumulated in the stalk region of fruiting bodies as second product of SteelyB enzyme. NMR and Crystal structural analysis revealed the detailed structure of LCC-1. The result of chlorine labelling experiment showed that ChlA, a chlorinating enzyme that regulated DIF-1 production was also involved in biosynthetic pathway of LCC-1. The feeding experiment indicated that THPH, a DIF-1 polyketide backbone rescued the production of LCC-1 in stlB null mutant. From these results, we hypothesized that SteelyB and ChlA are also involved in the LCC-1 biosynthesis. These two enzymes change their localization and thus change the modifying enzymes to produce LCC-1

Free Research Field

生物分子化学