Development of component technology for scanning microinjection microscope

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K04678
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Thin film/Surface and interfacial physical properties
• Research Institution: Kogakuin University
• Principal Investigator: Takami Tomohide 工学院大学, 教育推進機構(公私立大学の部局等), 教授 (40272455)
• Co-Investigator(Renkei-kenkyūsha): IWATA Futoshi 静岡大学, 学術院工学領域, 教授 (30262794) ; TAKAKUWA Yuji 東北大学, 多元物質科学研究所, 教授 (20154768)
• Research Collaborator: SUGAWARA Takeshi 広島大学, 大学院理学研究科, 特任助教 (60713005) ; TATE Shin-ichi 広島大学, 大学院理学研究科, 教授 (20216998) ; SAITO Mikako 東京農工大学, 大学院工学研究院, 准教授 (20291346) ; MATSUOKA Hideaki 東京農工大学, 大学院工学研究院, 名誉教授 (10143653)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: ナノピペット / 走査型プローブ顕微鏡 / 細胞工学 / イオン選別

Outline of Final Research Achievements

We have developed the following three element technologies to realize scanning microinjection microscope. (1) Motion control during the penetration of the pipette into the cell membrane during injection (2) Development of a non-destructive evaluating method of the cleanliness of the nanopipette inner wall by measuring the entrance pressure dependence of the vacuum conductance through a nanopipette set between two vacuum chambers in order to increase the accuracy of substance injection into cells during injection, and (3) Development of ion-selective nanopipette probe containing ion-selective membrane and establishment of the electrochemical etching of platinum electrode used in scanning pipette microscope.

Free Research Field

表面物理化学