2017 Fiscal Year Final Research Report
Construction of a Pipette Tip Tumor Marker Immunosensor with a Plasmonic chip based a Surface Plasmon Enhanced Fluorescence detection
Project/Area Number |
15K05540
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Analytical chemistry
|
Research Institution | Kobe University |
Principal Investigator |
Takano Eri 神戸大学, 工学研究科, 学術研究員 (20634645)
|
Co-Investigator(Kenkyū-buntansha) |
田和 圭子 関西学院大学, 理工学部, 教授 (80344109)
|
Project Period (FY) |
2015-04-01 – 2018-03-31
|
Keywords | イムノセンサ / 腫瘍マーカー / 表面プラズモン励起増強蛍光 / サンドイッチアッセイ |
Outline of Final Research Achievements |
A plasmonic chip base on grating-coupled surface plasmon field-enhanced fluorescence was prepared for the detection of tumor markers. A pipette tip-type immunosensor for tumor markers was constructed using the plasmonic chip, which was comprised of an automatic liquid handling system with fluorescence detection and a pipette tip designed to incorporate the plasmonic chip inside. A sandwich assay using this sensing system resulted in the detection of sub ng / mL target tumor markers within 20 minutes per a sample.
|
Free Research Field |
生体関連化学
|