Construction of a Pipette Tip Tumor Marker Immunosensor with a Plasmonic chip based a Surface Plasmon Enhanced Fluorescence detection

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05540
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Kobe University
• Principal Investigator: Takano Eri 神戸大学, 工学研究科, 学術研究員 (20634645)
• Co-Investigator(Kenkyū-buntansha): 田和 圭子 関西学院大学, 理工学部, 教授 (80344109)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: イムノセンサ / 腫瘍マーカー / 表面プラズモン励起増強蛍光 / サンドイッチアッセイ

Outline of Final Research Achievements

A plasmonic chip base on grating-coupled surface plasmon field-enhanced fluorescence was prepared for the detection of tumor markers. A pipette tip-type immunosensor for tumor markers was constructed using the plasmonic chip, which was comprised of an automatic liquid handling system with fluorescence detection and a pipette tip designed to incorporate the plasmonic chip inside. A sandwich assay using this sensing system resulted in the detection of sub ng / mL target tumor markers within 20 minutes per a sample.

Free Research Field

生体関連化学