Development of ultra sensitive CE-ESI-MS methods based on the nano-focusng stratedy

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K05546
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Gifu Pharmaceutical University
• Principal Investigator: ESAKA Yukihiro 岐阜薬科大学, 薬学部, 准教授 (70244530)
• Co-Investigator(Renkei-kenkyūsha): ISHIHAMA Yasushi 京都大学, 薬学研究科, 教授 (30439244) ; MURAKAMI Hiroya 愛知工業大学, 工学部, 准教授 (40515128) ; TORIMURA Masaki 国立研究開発法人産業技術総合研究所, イノベーション推進本部, 総括企画主幹 (40357588)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: CEオンライン濃縮 / オンライン錯体化 / 発がんリスクマーカー / 損傷ヌクレオチド / ESI-MS

Outline of Final Research Achievements

We have established a basis for highly sensitive detection of DNA damages occurred by drinking, developing capillary electorophoresis-mass spectrometry methods. The damaged bases were extracted from DNA as the corresponding 5’-nucleotides, and were enriched 3000-times by electrokinetic supercharging followed by on-line complexation with the Phostag reagent to enhance ESI efficiency of the nucleotides and finally were introduced to an ESI-MS system. The sensitivity would be more than 30,000-times higher compared with that with detections as the nucleotides by using a simple CE-ESI-MS method.

Free Research Field

CE・LCおよびMSをプラットフォームとする精密分離分析化学