2017 Fiscal Year Final Research Report
Optical inactivation of GluA1 homomeric receptors in a single dendritic spine.
| Project/Area Number |
15K06713
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neurophysiology / General neuroscience
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| Research Institution | Yokohama City University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 学習記憶 / 光操作 |
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| Outline of Final Research Achievements |
AMPA type glutamate receptors (AMPA-Rs) are known to have the important roles in synaptic activities and animal learning in vivo. AMPA-Rs are composed of variable combinations of four subunits, GluA1-4. In this project, to show the specificity of our GluA1 CALI technology, we found that CALI with Z9139 antibody showed the complex specificity to GluA1 homomer. We also elucidate the molecular specificity fo CALI with Z9139 in vivo using GluA1 KO mice. These results were published in Nature Biotechnology. We now conduct the CALI in singe spine on primary cultured neurons and acute hippocampal slices to elucidate the spatio-temporal function of GluA1 homomer.
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| Free Research Field |
神経科学
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