Establishment of an efficient gene knock-in method using adnoviral vector and CRISPR

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06807
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Yoshida Tetsu 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (00365438)
• Co-Investigator(Kenkyū-buntansha): 三谷 幸之介 埼玉医科大学, 医学部, 教授 (10270901) ; 汲田 和歌子 公益財団法人実験動物中央研究所, 応用発生学研究センター, 研究員 (50549128) ; 徳永 暁憲 国立研究開発法人国立長寿医療研究センター, その他部局等, 室長 (70549451)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: マーモセット / ノックイン

Outline of Final Research Achievements

Knock-in animals have been made by inducing gene targeting vector with a drug-resistant gene cassette into ES cells, followed by the drug selection. Next, chimera animals are generated using the knock-in ES cells, though the method can be applicable only for mice. Thus, we have to knock-in a gene in the genome of fertilized eggs to make knock-in marmosets. Since marmoset, a non-human primate model animal, ovulates fewer eggs than other animals, it is essential to get high rate of gene knock-in. Screening of the genes, including adenoviral coding genes, enhancing homologous recombination have been performed.

Free Research Field

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