Development of dataset of MALDI-TOF MS for bacteria derived from laboratory rodents

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06814
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Central Institute for Experimental Animals
• Principal Investigator: Hayashimoto Nobuhito 公益財団法人実験動物中央研究所, ICLASモニタリングセンター, センター長 (30332208)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: MALDI-TOF MS / 微生物学的品質管理

Outline of Final Research Achievements

Existing databases for microbial identification using MALDI - TOF MS are assumed to be used in human clinical test, and data of pathogens in experimental animals are insufficient. We expanded the database for the purpose of application to microbiological quality control of experimental animals. Mass spectral data including its closely related species, such as Pasteurella pneumotropica, Mycoplasma pulmonis, Corynebacterium kutscheri, etc., was acquired and registered in the database. As a result, it was possible to accurately identify strains that could not be identified in the default database, and we could build a system applicable to the site of microbiological quality control of experimental animals.

Free Research Field

実験動物感染症学