2017 Fiscal Year Final Research Report
Identification of Vasohibin binding proteins and their mechanisms
| Project/Area Number |
15K06821
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Tumor biology
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | Vasohibin / がん悪性化 / チューブリン脱チロシン化 / SVBP / がん関連線維芽細胞 / がん微小環境 |
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| Outline of Final Research Achievements |
In this study, I tried to identify the proteins associated with Vasohibin-2 (VASH2) and small vasohibin binding protein (SVBP), and analyzed their functions in tumor malignancy. I isolated and identified novel cell type specific proteins associated with VASH2 and/or SVBP using extracts of cancer cells, vascular endothelial cells, and mouse brain tissues. Point mutation analysis clarified important amino acid residues of VASH2 protein for binding to SVBP and for an alpha-tubulin detyrosinating activity of VASH2. Some of these mutants had dominant negative effects on the detyrosinating activity of endogenous VASH2 in cancer cells. Inhibition of VASH2 expression in cancer cells could attenuate the expansion of cancer-associated fibroblast in vivo and in vitro.
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| Free Research Field |
腫瘍生物学
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