Elucidation of the mechanism for regulating microenvironment and cancer development by mutant KRAS

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K06847
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Fukuoka University
• Principal Investigator: Tsunoda Toshiyuki 福岡大学, 医学部, 准教授 (70444817)
• Co-Investigator(Renkei-kenkyūsha): KOTAKE Yojiro 近畿大学, 産業理工学部, 准教授 (90531963) ; SHIRASAWA Senji 福岡大学, 医学部, 教授 (10253535) ; SASAKI Takamitsu 福岡大学, 医学部, 講師 (00382284)
• Research Collaborator: HIROSE Yumiko ; ISOYAMA Chiyo
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: KRAS / ３次元培養 / 低分子化合物

Outline of Final Research Achievements

In this study, we tried to find the compounds through the elucidation of the mechanism for regulating microenvironment and cancer development by the mutated KRAS. Three-dimensional (3D) floating culture system using the isogenic cell lines expressing wild-type KRAS or mutant (mt) KRAS were established from HKe3 cells, derived from colorectal cancer (CRC) HCT116 cells and disrupted at mtKRAS gene, and employed for the drug screening. During screening for 400 core-compounds from natural products which selectively suppress growth of human CRC spheroids with mtKRAS, we found 121 derivatives from 8 core-compounds and obtain a patent as inhibitors for signalling pathway associated with mtKRAS.

Free Research Field

分子腫瘍学