2017 Fiscal Year Final Research Report
Identification of apiosyltransferase involved in biosynthesis of pectin in plant cell wall
| Project/Area Number |
15K07016
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | 植物 / 細胞壁 / 多糖 / ペクチン / 糖転移酵素 / 糖ヌクレオチド / アピオース |
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| Outline of Final Research Achievements |
UDP-apiose, a donor substrate of apiosyltransferases, is known to a labile due to the intramolecular self-cyclization, resulting the formation of a-D-apio-D-furanosyl 1:2-cyclic phosphate. Its half-life is 32 seconds at at pH 8.0 and 25 C. We found that the bulky caions, especially tertiary amines, effectively suppressed the degradation of UDP-apiose. Consequently, a half-life of UDP-apiose was prolonged to 38.5 hours at pH 6.0 and 25C using triethylamine as a counter ion. Using this isolated UDP-apiose, we identified a gene encoding pectin rhamnogalacturonan II: apiosyltransferase.
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| Free Research Field |
生化学
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