Redox regulation of proteolysis in the Enterobacteriacea cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07019
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Nishii Wataru 国立研究開発法人理化学研究所, 横山構造生物学研究室, 研究員 (30287461)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: タンパク質分解 / 細胞内プロテオリシス / AAA+プロテアーゼ / レドックス / 腸内細菌科

Outline of Final Research Achievements

Cellular proteolysis dominates function, turnover, and quality of cellular proteins. Lon protease involves such proteolysis in bacterial cytosols and eukaryotic organella, and carries out a series of protein degradation processes within a single molecule. In this study, I investigated Lon protease by structural and functional analyses, including X-ray crystallography. The results showed that function of Lon protease is regulated through its dynamic structural changes in a multi-layered and stepwise manner. The results also shed light on the general mechanisms for cellular proteolysis, and provide opportunities to develop therapy, including that for infectious diseases.

Free Research Field

酵素学