Controlling the differentiation of hemogenic endothelial cells: Toward the derivation of hematopoietic stem cells from ES cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07081
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: OGAWA Minetaro 熊本大学, 発生医学研究所, 教授 (70194454)
• Research Collaborator: HIROTA Saeka , 大学院生 ; AHMED Tanzir , 大学院生
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 造血幹細胞 / 血管内皮細胞 / 造血性内皮細胞 / 中胚葉細胞 / ES細胞 / 試験管内分化 / 個体発生 / マウス

Outline of Final Research Achievements

Hematopoietic stem cells are generated from the hemogenic endothelium of the dorsal aorta. We developed an efficient method for inducing differentiation of hemogenic endothelial cells from mouse ES cells. We demonstrated that the differentiation potential of hemogenic endothelial cells can be controlled by extracellular signaling molecules. We established a culture system for inducing differentiation of hematopoietic stem cells from hemogenic endothelial cells isolated from mouse embryos. Even by using this culture system, however, we still failed to induce hematopoietic stem cells from ES cell-derived hemogenic endothelial cells. It is thus a future challenge to induce differentiation of genuine hemogenic endothelial cells from ES cells.

Free Research Field

造血発生学