Elucidation of the parasporin-1 receptor and effect of the anti-parasporin-1 receptor antibody to human cell lines

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07403
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Fukuoka Industrial Technology Center
• Principal Investigator: Okumura Shiro 福岡県工業技術センター, その他部局等, 専門研究員 (40399671)
• Co-Investigator(Renkei-kenkyūsha): KATAYAMA HIDEKI 福岡県工業技術センター, 生物食品研究所, 専門研究員 (90416496)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: パラスポリン / 受容体 / 細胞傷害活性 / がん

Outline of Final Research Achievements

The purpose of this study is to investigate the receptor of parasporin-1 (PS1) and investigate in detail the phenomenon that antibody against beclin-1 which is a potent receptor candidate induces apoptosis of cancer cells like PS1. For the search of receptors for PS1, we have searched with a photocrosslinking probe immobilized with PS1 and carried out analysis by western blotting, however, the reproducibility of the analysis result was not good. But we found that there is a correlation with the calcium concentration and the result of analysis. In addition, we produced recombinant beclin-1 by E. coli, and the monoclonal antibody was prepared from the protein. The cytotoxic activity against PS1-susceptible cell lines and the inhibitory ability against PS1 of the affinity purified antibody were measured, but neither activity was observed.

Free Research Field

応用微生物