Enantioseparation of aldohexoses and aldopentoses with L-tryptophanamide for detecting L-monosaccharides by reversed phase HPLC using HPLC

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07458
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Food science
• Research Institution: Tokai University
• Principal Investigator: Kodama Shuji 東海大学, 理学部, 教授 (70360807)
• Co-Investigator(Kenkyū-buntansha): 多賀 淳 近畿大学, 薬学部, 准教授 (20247951) ; 會澤 宣一 富山大学, 大学院理工学研究部(工学), 教授 (60231099) ; 山本 敦 中部大学, 応用生物学部, 教授 (60360806)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 光学異性体 / 単糖 / 高速液体クロマトグラフィー / 野菜

Outline of Final Research Achievements

D-Monosaccharides are distributed widely in nature, but it is not clarified whether L-monosaccharides, such as L-galactose, present in nature. We developed a novel reversed phase high-performance liquid chromatography for simultaneous enantioseparation of aldohexoses and aldopentoses derivatised with L-tryptophanamide. Using the above method, we tried to detect L-galactose in 8 vegetables. As a result, a small peak in only komatsuna sample was detected at the retention time of L-galactose. It is necessary to identify whether the peak is L-galactose in future.

Free Research Field

食品分析化学