Noninvasive method for detecting an invasive longicorn beetle in cherry trees

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07500
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Forest science
• Research Institution: Forest Research and Management Organization
• Principal Investigator: Shoda-Kagaya Etsuko 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (70353729)
• Research Collaborator: Tamura Shigeaki
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: クビアカツヤカミキリ / 外来生物 / DNA / PCR

Outline of Final Research Achievements

Frass suspected to have emitted by invasive beetle Aromia bungii from cherry trees were collected from seven locations. DNA was extracted using some molecular experiment kits. Mitochondrial DNA CO1 region was amplified with universal primers. Some kits were failed to extract DNA from frass. With an adequate method, we can successfully amplified 95% samples. Frass from none damaging area were shown to have emitted by other native longicorn beetles. We designed internal primers which are specific to A. bungii and the molecular method to identify A. bungii invasion using frass from a damaged Rosaceae trees was developed.

Free Research Field

森林科学