Molecular breeding of easily delignified plants by producing lignin carbohydrate complex (LCC)

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07509
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Wood science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: Kawai Shinya 東京農工大学, (連合)農学研究科(研究院), 准教授 (90202027)
• Co-Investigator(Renkei-kenkyūsha): FUNADA Ryo 東京農工大学, 大学院農学研究院, 教授 (20192734) ; YOSHIDA Makoto 東京農工大学, 大学院農学研究院, 教授 (30447510)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: リグニン / イネ / LCC分解 / 脱リグニン / エステラーゼ / アラビノフラノシダーゼ

Outline of Final Research Achievements

In order to increase the degradability of plant cell wall, we tried to cleave the linkages between lignin and carbohydrate in lignocarbohydrate complex (LCC) in planta. CcEST1 is an esterase of a white-rot-fungus, Coprinopsis cinerea. CcEST1 has both feruloyl-esterase activities. Thus, this enzyme can cleave bonds between the lignin and polysaccharide. And CcAbf62A is also an arabinofranosidase from C. cinerea. It can cleave the arabinofranosyl bonds in LCC. Because CcEST1 was a gene from a basidiomycete, the corresponding nucleotide sequence to a plant extracellular secretion signal was fused at the 5’-upstream site in frame to secrete effectively to the plant extracellular space. We analyzed the modified CcEST1 and CcAbf62A introduced transgenic rice plants. Transgenic rice plants produced CcEST1 contained slightly increased lignin contents, but lignin contents of ransgenic rice plants produced CcEST1 were not changed. In vitro digestibility of transgenic plants rised.

Free Research Field

植物遺伝子工学