Establishment of simple culture system for haploid embryonic stem cells

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07687
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Animal production science
• Research Institution: Gunma University
• Principal Investigator: Horii Takuro 群馬大学, 生体調節研究所, 准教授 (00361387)
• Co-Investigator(Kenkyū-buntansha): 畑田 出穂 群馬大学, 生体調節研究所, 教授 (50212147)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 半数体 / ES細胞 / FACS

Outline of Final Research Achievements

Haploid cells are useful for studying gene functions because disruption of a single allele can cause loss-of-function phenotypes. Recent success in generating haploid embryonic stem cells (ESCs) in mice, rats, and monkeys provides a new platform for simple genetic manipulation of the mammalian genome. Haploid ESCs tend to diploidize spontaneously due to endoreduplication or missed cytokinesis. It is therefore necessary to purify cells with haploid G1 DNA content at certain intervals. Supported by KAKENHI, we developed a novel haploid purification system that used a scatter plot generated by FACS analysis with a standard 488 nm laser.

Free Research Field

発生工学