2017 Fiscal Year Final Research Report
Clarification of Pathogenesis of AA amyloidosis via mutant SAA gene
| Project/Area Number |
15K07730
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Veterinary medical science
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| Research Institution | Azabu University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | AAアミロイド症 / 病理発生機序 / Serum amyloid A |
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| Outline of Final Research Achievements |
We demonstrated the presence of wild SAA and the mutant SAA in deposited amyloid in AA amyloidosis of dog, cat, cow and goat by mass spectrometry. An in vitro fibrillation assay demonstrated that the mutant SAA peptide formed typically rigid, long amyloid fibrils (10 nm wide), and the wild SAA peptide formed zigzagged, short fibers (7 nm wide). Moreover, the wild SAA peptide formed long, rigid amyloid fibrils in the presence of sonicated amyloid fibrils formed by the mutant SAA peptide. These findings indicate that the mutant SAA and wild SAA peptide mediate development of AA amyloidosis in animals via cross-seeding polymerization.
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| Free Research Field |
獣医病理学
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