Control of the proprerties of silk materials by the introduction of unnatural amino acids

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07800
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Insect science
• Research Institution: National Agriculture and Food Research Organization (2016-2017); 国立研究開発法人農業生物資源研究所 (2015)
• Principal Investigator: Teramoto Hidetoshi 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門 新産業開拓研究領域 新素材開発ユニット, 上級研究員 (60391562)
• Co-Investigator(Kenkyū-buntansha): 玉田 靖 信州大学, 学術研究院繊維学系, 教授 (70370666)
• Co-Investigator(Renkei-kenkyūsha): KOJIMA Katsura 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門新産業開拓研究領域新素材開発ユニット, 上級研究員 (40370655) ; NAKAJIMA Ken-ichi 国立研究開発法人農業・食品産業技術総合研究機構, 生物機能利用研究部門新産業開拓研究領域新特性シルク開発ユニット, 主席研究員 (70391563)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: バイオテクノロジー / 非天然アミノ酸

Outline of Final Research Achievements

Adhesion of fibroblasts (NIH3T3) on silk fibroin, incorporated with an azide-bearing unnatural amino acid (AzPhe), was investigated after being modified with cell adhesive RGD peptides or cell repulsive polyethylene glycols (PEG). When the RGD peptide was ligated on silk fibroin, fibroblasts with extended forms became abundant although number of adhered cells did not increase. When the PEG chain was ligated on silk fibroin, number of adhered cells significantly decreased. It was demonstrated that cell patterning on silk fibroin is possible by partially destroying azide groups by UV irradiation.

Free Research Field

応用昆虫学