Functions of secreted proteins to establish a symbiotic association with its host plant in fungal endophyte

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07839
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: Nagoya University
• Principal Investigator: Takemoto Aiko (田中愛子) 名古屋大学, 生命農学研究科, 学振特別研究員(RPD) (90464148)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 共生

Outline of Final Research Achievements

ProA is a transcription factor necessary for maintaining a symbiotic relationship with the host grass plants in Epichloe festucae. To identify ProA-binding sequences across the genome, we used a gSELEX method and collected the genomic DNA pools after gSELEX selection. The genomic DNA pools were sequenced using a Miseq platform. The direct target sequences were identified by mapping the reads to a reference sequence. Functional analysis of the potential new targets for ProA revealed 4 genes are encoding the proteins that contribute to maintaining the proper symbiotic interaction between E. festucae and the host ryegrass plants.

Free Research Field

農学