Generation and characterization of transgenic plants carrying hybrid chloroplasts

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07843
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Applied molecular and cellular biology
• Research Institution: University of Miyazaki
• Principal Investigator: INABA TAKEHITO 宮崎大学, 農学部, 准教授 (00400185)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 葉緑体 / 光合成 / シロイヌナズナ / 重炭酸イオン輸送体

Outline of Final Research Achievements

In this research, we aimed at installing cyanobacterial bicarbonate transporters into the inner envelope membrane of chloroplasts and creating "hybrid-chloroplast" carrying CO2-concentrating mechanism. We have successfully generated transgenic plants expressing chimeric cyanobacterial bicarbonate transporters (BicAI, BicAII, SbtAII, and SbtAIII). Furthermore, we have demonstrated that these chimeric bicarbonate transporters are localized to the inner envelope membrane of chloroplasts. We have also explored conditions to investigate bicarbonate transport activity using isolated chloroplasts.

Free Research Field

植物分子細胞生物学