2017 Fiscal Year Final Research Report
Diagnostic approach of diseases using non-invasive biosamples (saliva, hair and nail)
Project/Area Number |
15K07895
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical pharmacy
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Research Institution | University of Shizuoka |
Principal Investigator |
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Project Period (FY) |
2015-04-01 – 2018-03-31
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Keywords | 疾病診断 / 光学活性誘導体化試薬 / 非侵襲的試料 / バイオマーカー探索 / 液体クロマトグラフィー / 質量分析 |
Outline of Final Research Achievements |
Diagnostic approach of several diseases using non-invasive biological samples (saliva, hair and nail) was performed in this study. The determination of biomarker(s) related to diseases was based on targeted and non-targeted metabolomics by UPLC-MS. The chiral derivatization reagents, DMT-3(S)-Apy and DMT-L-Pro-OSu, were first synthesized for the determination of carboxylic acids and amines, respectively. The opposite enantiomer reagents and the isotopic variants were also synthesized. The ratio of D/L-lactic acids in the saliva of diabetic patients was higher than that of healthy persons. The ratio of polyamines in saliva was also different between breast cancer patients and healthy persons. Several N-acethyl amino acids in hairs of diabetes were significantly different from healthy subjects. Furthermore, the concentration of uric acid was obviously higher in gout patients. The efficiency of saliva, hair and nail as diagnostic sample was shown in this study.
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Free Research Field |
分析化学
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