Development of analytical method for glycoprotein-glycan interaction by hydrogen/deuterium exchange mass spectrometry

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K07916
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical pharmacy
• Research Institution: National Institute of Health Sciences
• Principal Investigator: Hashii Noritaka 国立医薬品食品衛生研究所, 生物薬品部, 室長 (20425672)
• Co-Investigator(Kenkyū-buntansha): 鈴木 琢雄 国立医薬品食品衛生研究所, 生物薬品部, 主任研究官 (10415466)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 水素重水素交換／質量分析 / 相互作用解析

Outline of Final Research Achievements

Protein glycosylation is involved in several biological functions via complex formation with target proteins and glycoproteins. To comprehensively understand biological roles of the glycan-glycoprotein complex, it is important to identify interaction sites in complexes as well as to reveal structural changes related to complex formation. However, many issues still remain in general higher-order structural analytical methods, including X-ray crystal structural analysis and NMR. These methods require complicated operations, such as crystallization and preparation of high-concentration sample solutions. We have analyzed protein-protein interactions under physiological conditions via hydrogen deuterium exchange reaction/liquid chromatography/mass spectrometry (HDX/MS). In this study, we evaluated the effectiveness of the HDX/MS using model glycan and glycoproteins. We demonstrated that HDX/MS is useful as an easy and rapid analytical method for glycan-glycoprotein interaction.

Free Research Field

分析化学