2017 Fiscal Year Final Research Report
Investigation of novel expression mechanism of HIV pathogenic protein to develop the next generation HIV therapy
| Project/Area Number |
15K08044
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Kumamoto University |
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Principal Investigator |
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| Project Period (FY) |
2015-04-01 – 2018-03-31
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| Keywords | HIV / Nef |
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| Outline of Final Research Achievements |
Nef is one of the accessory proteins of human immunodeficiency virus type 1 (HIV-1) and known as one of the virulence factors. Interestingly, the multiple-spliced Nef mRNAs have relatively long 5’ untranslated region (5’UTR), which are more than 700 nulceotides. We have previously reported that the 5’UTR of nef mRNA is suggested to contain a functional cis element for efficient Nef expression. We further investigated to characterize the cis element for efficient Nef expression in this study. As a result, it was suggested that the secondary structure of the cis element is associated with the efficient expression of Nef. It was also suggested that the cis element of 5’UTR functions for the efficient nuclear export of the nef mRNA. The efficient Nef expression was associated with the Nef function for enhancing the HIV-1 infectivity. These results strongly suggest that the cis element of 5’UTR of nef mRNAs is important for efficient Nef expression and HIV-1 replication.
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| Free Research Field |
ウイルス学
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