Long IRBIT regulates cell migration through modulating bicarbonate/chloride anion exchanger activity.

2017 Fiscal Year Final Research Report

• Project/Area Number: 15K08318
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Pathological medical chemistry
• Research Institution: Showa Pharmaceutical University
• Principal Investigator: HAMADA Koichi 昭和薬科大学, 薬学部, 講師 (00343070)
• Project Period (FY): 2015-04-01 – 2018-03-31
• Keywords: 細胞内ｐH

Outline of Final Research Achievements

[Purpose] Migration of cancer cells is one of critical parameters in metastasis. We found that more highly metastatic cells showed higher expression of long-IRBIT in both mouse and human melanoma cell lines, suggesting the potential role of long-IRBIT in metastasis. To examine the possibility, we searched for long-IRBIT binding proteins in highly metastatic melanoma cells, and tried to clarify the function of long-IRBIT and its binding protein in cell migration. [Result] A bicarbonate/chloride anion exchanger is identified as a binding protein of Long-IRBIT. Each melanoma cell knocked down for Long-IRBIT or the anion exchanger showed drastic decrease in cell migration ability. Activity of the anion exchanger is significantly reduced in Long-IRBIT knockdown cells. These results suggest that long-IRBIT positively modulates activity of the anion exchanger causing enhancement of cell migration.

Free Research Field

細胞生物学